PhIP can accurately identify known and previously unreported Coursework

PhIP throw out accurately identify known and previously unreported autoantigens - Coursework ExampleThe paper PhIP can accurately identify known and previously unreported autoantigens seeks to affirm the hypothesis that PhIP can accurately identify known and previously unreported autoantigens figure 3 shows seven figure that represent various experimental processes. Key technical issues in the program of work include the accurate and PhIP sequencing and T7 cloning, verifying and analyzing results from human samples with disparate consenting PND patients. Firstly the human protein and cDNA sequences available are downloaded from the database at build 35.1 of the human genome. Then this cDNA and the proteins are used to construct the library. This act involved the proteome wide library and the N/C terminal libraries that were PCR amplified as 23 independent pools with common primer sequences. This process was conducted in a controlled thermic profile. The polymerase chain reaction w as then digested and cloned into the T7FNS2 making numerous copies of each peptide.the cloning process involved the inserting a peptide in the required ORF sequence selected using rampart enzymes. This part is an a explanation of the processes involved in fig 3.d, g, f, c, e Patient A, B and C samples are collected from willing patients approved by relevant medical authorities. The samples of cerebrospinal fluids were kept at temperatures of - 80 degrees Celsius before being used. Before the collection of sample the patients have to undergo various neurologic evaluations before the samples.